Abstract:

Response surface methodology (RSM) was applied to optimize enzymatic hydrolysis of defatted silkworm pupa protein by commercial animal proteases. Effects of hydrolysis temperature, pH, protease amount and material-liquid ratio on the degree of hydrolysis (DH) were evaluated by single factor experiments. Results indicated that a linear relationship between hydrolysis temperature or protease amount and the degree of hydrolysis, a square effect of temperature, pH or material-liquid ratio on the degree of hydrolysis, cross-interaction of temperature and pH on the degree of hydrolysis were observed. The optimal hydrolysis condition for silkworm pupa protein was 45 ℃ of hydrolysis temperature, pH 7.56, 4.24% protease amount, and 1:29.7 of material-liquid ratio for 8 h of hydrolysis. The degree of hydrolysis of silkworm pupa protein was 48.24% under this optimal condition.

Key words: silkworm pupa protein, degree of hydrolysis, animal protease